Cat. No.
MABL-1155
Application
deplete, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
T11D7e
From
Recombinant Antibody
Specificity
This antibody binds murine and rat CD90. While CD90 is a monomorphic determinant in rats, CD90 is polymorphic in mice. As a result, this antibody reacts with Thy1.1-expressing mice, including AKR and FVB mice, but not Thy1.2-expressing mice, such as CBA and BALB/c.
Alternative Names
Thy-1; P01831; Thy1.1; Thy-1 membrane glycoprotein; Thy1; Thy-1 antigen
UniProt
P01830
Immunogen
This antibody was raised by immunising CBA mice with xenogeneic rat thymocytes, which express the Thy-1.1 antigen.
Application Notes
This antibody has been used in indirect immunofluorescence analysis of Thy-1 antigen expression in both methanol-fixed and living cell cultures (Dulbecco et al, 1979; Hasthorpe, 1980). This antibody has also been used, in conjunction with rabbit complement, to deplete Thy1-expressing fibroblasts from serum samples through complement-mediated lysis (Mahanthappa et al, 1996).
Antibody First Published
Lake et al. Production and characterization of cytotoxic Thy-1 antibody- secreting hybrid cell lines. Detection of T cell subsets. Eur J Immunol. 1979 Nov;9(11)：875-86. PMID：393521
Note on publication
Describes the original generation of this antibody as part of a panel of anti-Thy1.1 mAbs.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
