Cat. No.
MABL-1144
Application
IP, therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
14A8 (14.1)
From
Recombinant Antibody
Specificity
This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site.
Alternative Names
Immunoglobulin alpha Fc receptor; FCAR; IgA Fc receptor; FcαR
UniProt
P24071
Immunogen
The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.
Application Notes
This antibody binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.The binding of this antibody to human CD89 was confirmed with ELISA. This antibody was also capable of recognizing human CD89 expressing cells like human PMNL cells by flow cytometry. This antibody is not capable of blocking binding of IgA to CD89. This antibody can used to immunoprecipitate soluble CD89 (US7192582). A bispecific antibody construct incorporating the variable regions of the anti-gp41 antibody F240 and the anti-CD89 (immunoglobulin A [IgA] receptor) antibody 14A8 was reported to promote destruction of HIV type 1 (HIV-1) by neutrophils (PMID： 18272577). A Fab-like bispecific antibody molecule or Bi-Fab antibody generated from F240scFv and 14A8scFv showed significant antiboy dependent cell- mediated viral inhibition (ADCVI) activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection (PMID： 27419146).
Antibody First Published
Fishwild et al. High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice. Nat Biotechnol. (1996); 14(7)：845-51. PMID：9631008
Note on publication
Describes the generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
