Cat. No.
MABL-1142
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-48
From
Recombinant Antibody
Specificity
OX-48 binds specifically to CD86 targets which are present on subsets of T and B blasts, spleen macrophages and polymorphs, peritoneal macrophages, dendritic cells. OX48 does not appear to bind the same epitope of CD86 as 24F (Stephens et al. 2004). CD86 is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes - expression is rapidly upregulated by B cell specific stimuli. CD86 acts as a ligand for CD28 and CTLA-4. CD86 works in tandem with CD80 to prime T cells. 2 splice isoforms exist.
Alternative Names
B72; B-72; OX48; MRC OX-48; MRC OX48
UniProt
Q62810
Immunogen
OX-48 was prepared by immunizing mice with activated rat T cell blasts
Application Notes
OX-48 blocks IL-2 production and proliferation of T cells (except when in exponential phase) (Samoza et al. 1990). The antibody can also be used for FC (Stephens et al. 2004), FACS and IPs. Ox- 48 has been shown to be able to block binding of CD86 with its ligand CD28.
Antibody First Published
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol 24：1281 1987 PMID：2828930
Note on publication
Describes the use of Ox-48 among other anitbodies to identify new markers for activated T-cells, which might have expression related to theactive Vs quiescient state or may be involved in specific T-cell functions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
