Cat. No.
MABL-1132
Application
IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B7-24
From
Recombinant Antibody
Specificity
This monoclonal antibody recognizes human CD80 expressed on human blood dendritic cells and on in vitro activated (but not resting) B cells and monocytes.
Alternative Names
BB1; B7; B7-1; B7.1; CD28LG; CD28LG1; LAB7; Activation B7-1 antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80 ; CTLA4L; CTLA-4L
UniProt
P33681
Immunogen
The original antibody was generated by fusion with splenocytes from a mouse immunized with Sf9 insect cells expressing the human B7 molecule.
Application Notes
This mAb binds to a functionally important epitope of the B7 molecule. B7-24 is not able to inhibit T cell proliferation in primary mixed lymphocyte reactions where purified T cells are co- cultured with Epstein-Barr virus-transformed B cells. This antibody was used to study the cell surface expression of B7 antigen using flow cytometry. This antibody was also able to immunoprecipitate radioactive Iodine-labeled EBV-transformed B cells (PMID: 1280221). This antibody was used to examine the in situ expression of B7/BB1 by immunohistochemistry (PMID: 7682106).
Antibody First Published
Boer et al. Functional characterization of a novel anti-B7 monoclonal antibody. Eur J Immunol (1992); 22(12):3071-5. PMID:1280221
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
