Cat. No.
MABL-1127
Application
IP;IF;IHC, FC
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
#112
From
Recombinant Antibody
Specificity
This antibody is specific for chicken CD80, a type I glycoprotein consisting of two extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail. Functionally, CD80 on antigen presenting cells (APCs) binds to CD28 on T cells, thereby providing a costimulatory signal necessary for T cell activation.
Alternative Names
Cluster of Differentiation 80; CD80 protein; chicken CD80; chCD80
UniProt
Q5F3J1
Immunogen
The original antibody was raised by immunizing BALB/c mice with purified chicken CD80.
Application Notes
This antibody was used for detection of chicken CD80 expressed on CHO cells by flow cytometry; it was also used to detect CD80 on monocytes in the bursa of Fabricius and on spleen monocytes. chCD80 from transfected CHO cells, or from LPS-stimulated mononuclear lymphocytes were used for immunoprecipitation. In this procedure the protein was immunoprecipitated using this antibody.This antibody was used for immunohistochemistry on the bursa of Fabricius and spleen. Immunofluorescence was performed on chicken intestinal dendritic cells using this antibody. The antibody inhibited ConA-stimulated spleen cell differentiation into MHCII+ cells and blocked IL-2 induced spleen blast cell proliferation (Lee et al, 2011; pmid: 21334748).
Antibody First Published
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken CD80. Comp Immunol Microbiol Infect Dis. 2011;34(3):273‐279 PMID:21334748
Note on publication
The paper describes the generation and characterization of the antibody, which is specific for chicken CD80.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
