Cat. No.
MABL-1103
Application
IP, RIA, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LL1 (EPB1)
From
Recombinant Antibody
Specificity
This antibody reacts with the cell surface epitope of the invariant chain (Ii) subunit of the immatrue MHC class-II antigen CD74.
Alternative Names
Ii; p33; DHLAG; HLADG; Ia-GAMMAHLA; HLA Class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain;
UniProt
P04233
Immunogen
This antibody was generated by immunizing female BALB/c mice with whole Raji cells or a crude membrane preparation.
Application Notes
Radioimmunobinding and flow cytometric analysis was used to analyse binding of LL1 to peripheral blood. LL1 did not react with T cell lines nor with normal diploid cells. The reactivity of LL1 (EB- 1) to the tumor cells was checked using a live cell indirect immunoflurescence staining assay. LL1 stained positive for cell lines Raji (maxilla), Daudi (B-lymphoblast), RAMOS (b-lymphocyte), SU-DHL-7, ARH-77 (plasma cell leukemia), HS-Sultan (plasmacytoma). LL1 is reported to recognize most of the small and large lymphocytes of the germinal centre and no reactivity to marginal zone B-cells. It is also reported to react with interdigitating histiocytes of the T-cell zones. It was further reported that LL1 bound positively to Western blotted Raji cell antigens (PMID: 2663143). The specificity of LL1 for Ii subunit was determined by immunoprecipitation. Radiolabelled antibodies were used for internalization and catabolism of the antibody and it was seen that LL1 was internalized and catabolized very quickly as compared to some other antibodies like those which are specific for CD19, CD22 and anti-transferrin receptor. (PMID: 8947500). LL1 was evaluated to be a potential candidate for bone marrow imaging using Tcm labelled LL1 sulphur colloid imaging. (PMID: 9076770)
Antibody First Published
Pawlak-Byczkowska et al. Two New Monoclonal Antibodies EPB-1 and EPB-2 Reactive with Human Lymphoma1. Cancer Research (1989); Volume 49; pg 4568-4577. PMID:2663143
Note on publication
Describes the generation and immunohistochemical characterization of antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
