Cat. No.
MABL-1101
Application
in vitro, IP, RIA, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BU45
From
Recombinant Antibody
Specificity
This antibody is specific for the lumenal portion of human CD74.
Alternative Names
li; Ia antigen-associated invariant chain; HLA-DR antigens-associated invariant chain; HLA class II histocompatibility antigen gamma chain; invariant chain
UniProt
P04233
Immunogen
The original antibody was raised by immunizing a mouse with B lymphoblastoid cell line, HFBl.
Application Notes
The original version of this antibody was used for flow cytometry on single cell suspensions from the spleen (Genève et al., 2014; PMID:25171001), and on (Wraight et al., 1990; PMID:1690714), and on gastric epithelial cells (Barrera et al., 2005; PMID:15923369). This antibody was used for immunofluorescence on transfected human fibroblast cell lines (M1DRIi) (Stang and Bakke., 1997; PMID:9281355), on Stably transfected MDCK cells (Simonsen et al., 1998; PMID:9650780) and on B cells (Roche et al., 1993; PMID:8397411). Furthermore, the antibody was used for immunoprecipitation on class Il-negative B x T hybrid cell line T2 and its class II positive parental cell line T1 (Henne et al., 1995;
PMID:7750992), on U937 cell lysates (Wraight et al., 1990; PMID:1690714), on stably transfected MDCK cells grown (Simonsen et al., 1998; PMID:9650780), and on gastric epithelial cells (Barrera et al., 2005; PMID:15923369). This antibody was used for an radioimmunoassay on a JOK-1 B cell line, Raji Burkitt’s lymphoma, and U937 cells. To determine the binding sites of this antibody on the surface of the cells (Wraight et al., 1990; PMID:1690714). This antibody was used for an internalization assay on stably transfected MDCK cells where it bound to the surface (Simonsen et al., 1998; PMID:9650780).
Antibody First Published
Wraight et al. Human major histocompatibility complex class II invariant chain is expressed on the cell surface Biol Chem. 1990 Apr 5;265(10):5787-92. PMID:1690714
Note on publication
The original paper describe a study with three monoclonal antibodies which recognize an extracytoplasmic determinant present on all forms of the invariant chain and use them to demonstrate its presence on the surface of the intact cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
