Cat. No.
MABL-1099
Application
inhibit, IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1E 9
From
Recombinant Antibody
Specificity
This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)- linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73.
Alternative Names
NT5E; 5'-NT; 5'-nucleotidase; Ecto-5'-nucleotidase
UniProt
P21589
Immunogen
The original antibody was generated by immunizing mice with highly purified human placental 5'-NT as immunogen.
Application Notes
The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody inhibits soluble placental 5'-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. This antibody was also used in the study of cellular distribution of CD73 (PMID: 2137649). It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab')2 goat anti-mouse Ig (PMID: 2137649, 2550543). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). This antibody was also used to detect CD73 in COS cells transfected with CD73 cDNA using flow cytometry (PMID: 7595232). Flow cytometry analysis also showed that anti-CD73 1E9 mAb recognized CD73 on the human breast cancer cell lines LM3 and MDA-MB-231, but failed to detect the murine CD73 variant expressed on isolated mouse mononuclear hematopoietic cells(PMID: 24043904). Bispecific antibody generated using 1E9 and mAb AD2 is reported to inhibit the enzymatic activity of CD73 as well as promotes internalization of CD73.
Antibody First Published
Thomson et al. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens (1990); 35(1):9-19. PMID:2137649
Note on publication
Describes the generation and characterization of this antibody and its use in studying the expression of CD73 on various types of blood lymphocytes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
