Cat. No.
MABL-1097
Application
Activate, stimulatory, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BU40
From
Recombinant Antibody
Specificity
This antibody is specific for CD72, which is highly expressed by pre-B cells and B-cells, but not terminally differentiated plasma cells.
Alternative Names
Lyb-2; B-cell differentiation antigen CD72
UniProt
P21854
Immunogen
This antibody was raised against the HF B1 plasmacytoid cell line, which is considered to be late B-like.
Application Notes
This antibody has been used to investigate cell-surface expression of CD72 in FACS analysis (Kataoka et al, 2013). This antibody has been shown to induce resting B cells to enter the G1 phase of the cell cycle, stimulate B cell expression of major histocompatibility complex class II antigen and potentiate the capacity of IL-4 to induce CD23 production (Katira et al, 1992). It also stimulates TNF-α and IL-6 production in CD14+ monocytes (Yoshida et al, 2015). Ligation of CD72 by this antibody has been observed to suppress the proliferation of an acute myelogenous leukaemia cell line (Kataoka et al, 2013).
Antibody First Published
Van de Velde et al. The B-cell surface protein CD72/Lyb-2 is the ligand for CD5. Nature. 1991 Jun 20;351(6328):662-5. PMID:1711157
Note on publication
Describes the use of this antibody to block the CD5-CD72 interaction
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
