Cat. No.
MABL-1084
Application
FACS, IP, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BU69
From
Recombinant Antibody
Specificity
This antibody binds human CD70, which is the CD27 ligand. This protein is present only on activated dendritic cells and T lymphocytes, and represents a therapeutic target of relevance for autoimmune diseases.
Alternative Names
TNFRS7; Tumor Necrosis Factor Ligand Superfamily Member 7; CD27 Ligand; CD27LG; TNFSF7; CD27L; Tumor Necrosis Factor Ligand 8A; Surface Antigen CD70; Ki-24 Antigen; CD70 Antigen; TNLG8A; TAN1-7; TAN17
UniProt
P32970
Immunogen
Murine antibodies were produced by immunising BALB/c mice using the Waldenstrom's macroglobulinaemia cell line WM-1. Hybridomas were generated by fusion of immunised cells with the myeloma cell line P3/NS1/1-Ag4.1 via established methods.
Application Notes
BU69 has been used for FACS and immunocytochemistry analysis of the cell-surface expression levels of CD70 in renal cell carcinomas (Adam et al, 2006). It was shown in this study that cell- surface binding of BU69 to CD70 caused rapid internalisation of the cell-surface glycoprotein.
Antibody First Published
Edited by Schlossman et al. Leukocyte typing V: White cell differentiation antigens Oxford
Note on publication
Describes the repertoire of leukocyte differentiation antigens (i.e. Clusters of Differentiation, and other such cell-surface molecules), specifically using a panel of mAbs to then facilitate characterisation of distinct cell types based on their antibody reactivity patterns.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
