Cat. No.
MABL-1082
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
3H4
From
Recombinant Antibody
Specificity
This antibody binds human and cynomolgus monkey CD70, a cytokine belonging to the tumor necrosis factor (TNF) ligand family, which acts as a ligand for TNFRSF27/CD27. It is a surface antigen present on activated T and B cells. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. CD70 is also identified as an emerging target in cancer immunotherapy.
Alternative Names
TNFSF7; CD27L; CD27-L; CD27LG; CD27 ligand; CD70 antigen; Tumor necrosis factor ligand superfamily member 7
UniProt
P32970
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant human CD70-mFc fusion protein as immunogen.
Application Notes
The binding this antibody to recombinant human CD70 was determined using ELISA. This antibody can positively react with human and cynomolgus monkey CD70 expressed on the surface of 786-0 renal carcinoma cells and CHO-K1-cmCD70 cells in a flow cytometric assay (CN110724670).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
