Key features and details | |
Cat. No. | MABL-853 |
Name | Anti-CD28 mAbs |
Clone No. | AFD-9.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, IP, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for human CD28. CD28 is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins. |
Alternative Name | T-cell-specific surface glycoprotein CD28; TP44 |
UniProt | P10747 |
Immunogen | The original antibody was generated by immunizing mice with human T cells. |
Application Notes | The antibody was found to induce T cell activation and proliferation in collaboration with TPA. Further, T cell proliferation was associated with IL-2 receptor expression and IL-2 production. The antibody stained 60-80% of the isolated T cells by immunofluorescence. The antibody immunoprecipitated CD28 from Jurkat cells. (Hara et al., 1985; PMID: 3159820). Immunofluorescence was performed on T cells expressing CD28 using this antibody. The antibody was cytotoxic for 50-80% of peripheral T cells and for 20- 50% of thymocytes but it was not cytotoxic for cultured B-cell lines, normal B cells, or monocytes. In immune precipitation assays, the antibody reacted with CD28 (Hansen et al., 1980). The antibody induces proliferative responses in human T cells in in vitro experiments (Hutloff et al. 1999; PMID: 9930702). The antibody detected CD28 by western blot analysis (Siefken et al,1998; PMID: 9712026). Humanize, chimeric and hybrid Fab versions of the antibody were constructed. |
Antibody First Published | Hansen et al. Monoclonal antibodies identifying a novel T-Cell antigen and Ia antigens of human lymphocytes Immunogenetics. 1980; 10:247. |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
