Cat. No.
MABL-1080
Application
ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
YTH3.2.6
From
Recombinant Antibody
Specificity
This antibody binds CD7.
Alternative Names
GP40; LEU-9; TP41; Tp40; T-cell leukemia antigen; P41 protein; YTH3.2.6AG5
UniProt
P09564
Immunogen
The k-secreting myeloma line Y3/Ag1.2.3 was fused with spleen cells from DA rats immunised with normal human lymphocytes.
Application Notes
The fraction of human lymphocyte cells recognised by YTH 3.2 was analysed by flow cytometry, using fluorescein-labelled F(ab')2 fragments of rabbit anti-(rat Ig) to stain the antibody. Binding has also been characterised by ELISA. In preliminary tests, graft rejection in patients with steroid-resistant kidney rejection indicated that graft rejection could be reversed by YTH 3.2 injections (Hale et al, 1985). This antibody has been used to stain T-cell dependent areas in immunohistology (King et al, 1989).
Antibody First Published
Hale et al Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol 1985; 134:3056-3061 PMID:3980990
Note on publication
Describes the generation and analysis of rat monoclonal antibodies for use in serotherapy.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
