Cat. No.
MABL-1077
Application
IP, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
T3-3A1
From
Recombinant Antibody
Specificity
This antibody binds human CD7. CD7 is expressed on the majority of immature and mature T- lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. CD7 is useful in the identification of precursor T cells as well as an important marker for the identification of leukemic T cells.
Alternative Names
GP40; TP41; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9 ; T3.3A1; 3A1
UniProt
P09564
Immunogen
The original antibody was generated by immunizing female BALB/c mice both intravenously and intraperitoneally with HSB-2 T cells.
Application Notes
This antibody is capable of recognizing CD7 antigen present on various T cell lines like n HSB-2, HPB-MLT, MOLT-4, CEM, and TALL-1. This antibody also bound a majority (85%) of human PB E- rosette-positive cells. Immunoprecipitation of an HSB-2 extract with this antibody yielded one specific band with a molecular weight of approximately 40,000 in the presence of reducing agent (PMID: 6156457). This antibody was also used in the development of a cell ELISA to monitor the PHA-induced expression of the CD7 antigen in fixed mononuclear leukocytes (PMID: 3492562). This antibody was also used in combination with another anti-CD7 antibody mab 69 of development of a sandwich ELISA for detection of solubilized CD7 antigen (PMID: 2464646). This antibody was used in a study relating to identifying CD7 as target structure for T-cell imaging. Noninvasive CD7 immuno-imaging is a promising strategy to track T-cell responses in the context of diverse immunotherapies in vivo with additional theragnostic potential in T-cell-mediated diseases (PMID: 30613283).
Antibody First Published
Haynes et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc Natl Acad Sci U S A. 1979 Nov; 76(11): 5829–5833. PMID:316538
Note on publication
Describes the generation of this antibody and uses it for the study of CD7 positive and negative T cell subsets in the regulation of normal and abnormal human immune responses.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
