Cat. No.
MABL-1075
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3A1f
From
Recombinant Antibody
Specificity
This antibody binds human CD7. CD7 is expressed on the majority of immature and mature T- lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. CD7 is useful in the identification of precursor T cells as well as an important marker for the identification of leukemic T cells.
Alternative Names
GP40; TP41; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; 3aIf
UniProt
P09564
Immunogen
Application Notes
The binding affinity of the scFv version of this antibody was determined using surface plasmon resonance. The affinity of the scFv antibody for human CD7 was found to be 1.8 nM. This the scFv version of this antibody was also used in the generation of single-chain Fv (scFv) immunotoxins specific for the T cell-associated antigen CD7 as a novel therapeutic agent for treatment of human T cell malignancies (PMID: 9120282). This antibody was also used in the generation of a therapeutic fusion protein scFvCD7:sTRAIL, designed to induce target antigen-restricted apoptosis in human T-cell tumors. The binding of the fusion protein to CD7 was tested with flow cytometry (PMID: 15833872).
Antibody First Published
Pauza et al. Construction and characterization of human CD7-specific single- chain Fv immunotoxins. J Immunol. 1997 Apr 1;158(7):3259-69. PMID:9120282
Note on publication
Describes the generation of scFv version of this antibody for the construction of single-chain Fv (scFv) immunotoxins .
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
