Cat. No.
MABL-1066
Application
competition studies, toxicity assays, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
H22
From
Recombinant Antibody
Specificity
This antibody specifically targets human CD64, recognizing an epitope distinct from the Fc binding domain.
Alternative Names
FcγRI; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA
UniProt
P12314
Immunogen
The original antibody was generated by cloning a single-chain fragment (scFv) derived from the murine anti-human CD64 monoclonal antibody (mAb) clone "m22". Clone m22 was originally described by Guyre et al. (1989).
Application Notes
This antibody can be bound to a toxin so that it displays specific cytotoxicity and thus can be used to eliminate AML cells selectively. This antibody was bound to a truncated version of the Pseudomonas exotoxin A (ETA'). The binding activity of the VH/VL antibody format was unaffected by the fusion of the m22(scFv) coding regions to the ETA' coding sequences. FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur et al., 2003; PMID: 14679004). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur et al., 2011; PMID: 21077160).
Antibody First Published
Tur et al., Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function & Recombinant CD64-Specific Single Chain Immunotoxin Exhibits Specific Cytotoxicity against Acute Myeloid Leukemia Cells Journal of immunology, 1 September 1989, Vol.143(5), pp.1650-5 & CANCER RESEARCH 63, 8414 – 8419, December 1, 2003 PMID:14679004
Note on publication
The original article by Tur et al. (2003; PMID: 14679004) described the development of an immunotoxin-conjugated recombinant anti-CD64 monoclonal antibody and demonstrated its efficacy in targeting CD64+ acute myeloid leukemia cells, inducing apoptosis. The parental murine antibody clone "m22", used to generate clone H22, was first reported by Guyre et al. (1989; PMID: 2474608). It was employed in FC, IP, staining, cytotoxicity assays, and cross-blocking analyses to characterize FcγRI epitopes, showing its ability to trigger cytotoxicity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
