Cat. No.
MABL-1028
Application
IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
YKIX 322.3
From
Recombinant Antibody
Specificity
YKIX 322.3 binds specifically to CD5. CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein present on all mature T lymphocytes, on most thymocytes, and a subset of B lymphocytes (and on many T cell leukemias and lymphomas), but not on natural killer (NK) cells. CD5 is the major ligand of the B cell antigen CD72. Binding of CD5 on the T cell surface can augment alloantigen or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion and IL2R expression. CD5 is also thought to negatively regulate signal transduction mediated by the T cell and B cell receptors.
Alternative Names
LEU-1; LEU1; Ly-1; Lymphocyte antigen T1/Leu-1; Lyt-1; T-cell surface glycoprotein CD5
UniProt
F1PJU1
Immunogen
YKIX 322.3 was prepared by immunizing rats with concanavilin A activated canine peripheral blood cells.
Application Notes
YKIX 322.3 can be used for IHC-Fr and can act as a marker for T cells in addition to being used for IP.
Antibody First Published
Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414
Note on publication
Describes the binding specificity and characterisation (by IF staining and FACS, and IP) of antibodies against canine equivalents of human CD antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
