Cat. No.
MABL-1024
Application
functional assay, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
5D7
From
Recombinant Antibody
Specificity
This antibody binds human CD5, which is a surface glycoprotein that act as a receptor in regulating T-cell proliferation. CD5 is a good immunohistochemical marker for T-cells. This antibody also cross reacts with cynomolgus monkey CD5.
Alternative Names
LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1
UniProt
P06127
Immunogen
Application Notes
The binding of this antibody to human and cynomolgus monkey PBMCs was studied using flow cytometry. The human IgG1 version of this antibody mediates significant ADCC activity against the CLL cell line Wac3CD5, using purified NK cells as effector cells. Anti-RSV IgGI was used as an isotype control. In a caspase activity assay this antibody showed capability of inhibiting survival of both CLL and ALCL tumor cells by mediating apoptosis against these cells. Humanized version of this antibody is available on request (US20080254027).
Antibody First Published
Stöckl et al. Leukosialin (CD43)-major histocompatibility class I molecule interactions involved in spontaneous T cell conjugate formation. J Exp Med. 1996 Nov 1; 184(5)： 1769–1779. PMID：8920865
Note on publication
This paper investigates the molecular interactions involved in spontaneous T cell conjugate formation using various antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
