Cat. No.
MABL-1012
Application
IP, WB, Block, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-101
From
Recombinant Antibody
Specificity
OX-101 binds specifically to CD47 and has been shown to compete with OX-41 for binding . CD47 acts as an adhesionn receptor for THBS1 on platelets and is involved in the modulation of integrins (signalling). Acts as a SIRPA receptor - SIRPA binding prevents maturation of immature dentritic cells and and inhibits cytokine production by mature dendritic cells. Possibly involved in changes to membrane permeability due to virus infection.
Alternative Names
IAP; OX101; MRC OX-101; MRC OX101; leukocyte surface antigen CD47; integrin-associated protein; intergin associated protein
UniProt
P97829
Immunogen
OX-101 was prepared by immunizing BALB/c mice with biotinylated rat thymocytes - this antibody was selected from a number of antibodies raised by the immunogen.
Application Notes
This antibody can be used for affinity chromatography purification and can block the interaction between OX41-CD4d3+4 beads and cells. In addition OX-101 can be used for FC and WB.
Antibody First Published
Vernon-Wilson et al CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1. Eur J Immunol 30:2130-7 2000 PMID:9712053
Note on publication
Describes the use of OX-101 in identifying CD47 as the ligand for SIRP.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
