Cat. No.
MABL-1004
Application
IP, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B6H12
From
Recombinant Antibody
Specificity
This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Alternative Names
IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker
UniProt
Q08722
Immunogen
The original antibody was generated by immunizing BALB/c mice with purified placental RGD- binding proteins (PRBP).
Application Notes
This antibody reacts with placental RGD-binding proteins and human neutrophils in an ELISA (PMID: 2785522). This antibody binds CD47 expressed on human neutrophils in a flow cytometric assay. This antibody immunoprecipitated vitronectin receptor (VnR) from detergent-solubilized placental membrane preparations (PMID: 2785522). It is a function-blocking antibody that inhibits CD47 interactions with TSP1, SIRPα, some integrins, and EGF receptor (PMID: 19636412; 10572074; 19004835; 26840086).This antibody directly induces killing of tumor cells by NK cells, B cell migratiom, and regulatory T cell differentiation (PMID: 18497546; 10891456; 16951312). B6H12 enhanced antibody-dependent cellular phagocytosis (ADCP) of human acute myeloid leukemia (AML) cells in vitro by human and mouse macrophages (PMID: 19632179). B6H12 was used in several preclinical studies to explore combining CD47 blockade with therapeutic antibodies that target specific cancers. This antibody in combination with CD20 antibody rituximab, to promote phagocytosis in vitro and eradicated non-Hodgkin lymphoma in NSG mice (PMID: 20813259).
Antibody First Published
Gresham et al. A novel member of the integrin receptor family mediates Arg-Gly-Asp-stimulated neutrophil phagocytosis. J Cell Biol. 1989 May;108(5):1935-43. PMID:2785522
Note on publication
Describes the generation of this antibody and its use in determining the role of CD47 in Arg-Gly-Asp-stimulated neutrophil phagocytosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
