Cat. No.
MABL-994
Application
IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-22
From
Recombinant Antibody
Specificity
OX-22 binds specifically to the high molecular weight form of the leucocyte common antigen, CD45RC. OX-22 and OX-31 compete for binding but do not compete with OX-32. The antigen is expresssed on the surface of B cells, around half of bone marrow cells, all CD8+ve T cells, and is able to differentiate between CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like) cell types. CD45 extracellular domain is able to interact with a number of different proteins including CD4, TCR, CD2, Thy-1 and CD26.CD45 is important for signalling throguh the T cell receptor and in the selection of T and B lymphocytes.
Alternative Names
L-CA; Receptor-type tyrosine-protein phosphatase C; leukocyte common antigen; T200; CD45; OX22; MRC OX22; MRC OX-22; CD45 R
UniProt
P04157
Immunogen
OX-22 was prepared by immunizing BALB/c mice with phytohemagglutinin-activated rat lymph node cells.
Application Notes
OX-20 can be used for both FC and IHC (paraffin/frozen) studies. It can also be used to purify proteins by affinty-purification (Woolett et al. Eur J Immunol. 1985 Feb;15(2):168-73).
Antibody First Published
Spickett et al. MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen. J Exp Med. 1983 Sep 1;158(3):795-810. PMID:6224884
Note on publication
Describes the use of OX-22 to analyse CD45RC tissue distribution and antigen characterisation which enable the identification of distinct subsets of T cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
