Cat. No.
MABL-973
Application
FACS, IP, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Dog, Raccoon
Clone No.
YKIX 337.8.7
From
Recombinant Antibody
Specificity
YKIX 337.8.7 specifically binds to CD44. CD44 has high affinity for hyaluronic acid (HA) and can mediate cell-cell and cell-matrix interactions through its affinity for HA (and possibly also osteopontin, collagens, and matrix metalloproteinases (MMPs)). Adhesion with HA is important for cell migration, tumor growth and progression. CD44 also plays a role in lymphocyte activation, recirculation and homing, and in hematopoiesis. CD44 is a receptor for LGALS9 - this interaction enhances SMAD3 binding to the FOXP3 promoter, up-regulating FOXP3 expression and increasing induced regulatory T (iTreg) cell stability and suppressive function.
Alternative Names
HCAM; CD44 antigen; extracellular receptor III; ECMR III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; HUTCH I; HUTCH 1; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; PGP-1; PGP 1; PGP I
UniProt
Q28284
Immunogen
YKIX 337.8.7 was prepared by immunizing rats canine thymocytes.
Application Notes
YKIX 337.8.7 antibody can be used for IHC-Fr; YKIX 337.8.7 to distinguish CD4+ T-cell subsets. This antibody can additionally be used for FACS and IP.
Antibody First Published
Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414
Note on publication
Describes the binding specificity and characterisation (by IF staining and FACS, and IP) of antibodies against canine equivalents of human CD antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
