Cat. No.
MABL-950
Application
catalysis, crystallography, in vivo, IP, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5c8
From
Recombinant Antibody
Specificity
This antibody is specific for human CD40 ligand.
Alternative Names
CD40LG; TRAP; CD154; CD40 ligand; CD40L; TNFSF5, tumor necrosis factor ligand superfamily member 5; tumor necrosis factor related activation protein
UniProt
P29965
Immunogen
The original antibody was raised by immunizing a BALB/c mouse with D1.1 cells.
Application Notes
The original version of this antibody was used to catalyse disfavoured endo-tet cyclization reactions of trans-epoxy alcohols (Gruber et al., 1999; PMID:10353817). The structure of the fab version of the antibody was determined using x-ray crystallography (Gruber et al., 1999; PMID:10353817, Karpusas et al., 2001; PMID:11525169). The original antibody was used for flow cytometry on D1.1 cells. It only showed binding to D1.1 cells but not to B2.7 cells. This antibody was used for immunoprecipitation on Jurkat cell lysates and precipitated a 30kDa protein. This antibody also shows the ability to inhibit D1.1 cell induced B cell activation (Lederman et al., 1992; PMID:1348081). The half life of this antibody in cynomolgus monkeys was measured. The data suggested a half life between 300-500 hours. This antibody was tested in vivo on cynomolgus monkeys. When first given this antibody and then performing a tetanus toxoid challenge it decreased antibody titres (Gobburu et al., 1998; PMID:9694951).
Antibody First Published
Lederman et al. Identification of a novel surface protein on activated CD4+ T cells that induces contact-dependent B cell differentiation (help) J Exp Med. 1992 Apr 1;175(4):1091-101. PMID:1348081
Note on publication
To identify T cell surface molecules that mediate contact-dependent T helper function, the original paper generated a monoclonal antibody (mAb), designated 5c8, that inhibits D1.1- mediated B cell activation and immunoprecipitates a novel 30-kD protein structure from surface-iodinated D1.1 cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
