Cat. No.
MABL-944
Application
Activate, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A54 scFv
From
Recombinant Antibody
Specificity
A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact, i.e., non- truncated version of CD40, and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). The scFv version of this antibody binds CD40 (immobilized by amine coupling) with a KD of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells, and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily, and this interaction is required for the seconfary immune responses and germinal centre formation.
Alternative Names
TNFRSF5; A54; Tumor necrosis factor receptor superfamily member 5; B cell surface antigen CD40; B-cell cell surface antigen CD40; B-cell surface antigen CD40; B cell surface antigen CD40; antigen CD40; CD40 antigen; Bp50; Bp-50; CD40L receptor CDw40
UniProt
P25942
Immunogen
A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcγ fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Application Notes
A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)
Antibody First Published
Ellmark et al. Modulation of the CD40-CD40 ligand interaction using human anti-CD40 single-chain antibody fragments obtained from the n-CoDeR phage display library Immunology . 2002 Aug;106(4):456-63. doi: 10.1046/j.1365-2567.2002.01473.x PMID:12153507
Note on publication
The original publication describes the selection and characterization of a novel set of fully human anti-CD40 single-chain antibody fragments obtained from a phage display library.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
