Cat. No.
MABL-922
Application
binding assay, IP, SPR, WB, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-68
From
Recombinant Antibody
Specificity
This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.
Alternative Names
Leu3/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu- 3; W3/25 antigen
UniProt
P05540
Immunogen
This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.
Application Notes
This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50% reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.
Antibody First Published
Simon et al. Role of CD4 epitopes outside the gp120-binding site during entry of human immunodeficiency virus type 1. J Virol. 1997 Feb;71(2):1476-84. PMID:8995673
Note on publication
Describes the original use of this antibody to elucidate the role of CD4 epitopes outside the gp120-binding site during HIV-1 entry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
