Cat. No.
MABL-831
Application
WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
32
From
Recombinant Antibody
Specificity
The antibody specifically binds to chicken CD25, a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also called interleukin-2 receptor alpha chain, and together with beta chains and common gamma chain, constitute the high-affinity IL2 receptor.
Alternative Names
IL-2Rα; Interleukin-2 receptor subunit alpha; Interleukin-2 receptor alpha chain; chCD25; chCD25-32
UniProt
Q90WJ4
Immunogen
The original antibody was raised by immunizing BALB/c mice with recombinant chicken CD25 protein.
Application Notes
The original antibody detected the recombinant chicken CD25 by western blot from Con A-stimulated spleen cells. The original antibody detected CHO cells stably transfected with chCD25/IgG4 by flow cytometry. Furthermore, the antibody stained HD11 chicken macrophages, spleen mononuclear cells, and bursa derived lymphocytes. The antibody stained of MDV-transformed T cell lines, UD35 and US53, reticuloendothelial virus-transformed B cell line, CU60, and avianleukosis virus transformed macrophage cell line, HTC. This antibody was used in immunofluorescence in different tissues (spleen, bursa of Fabricius, and intestinal duodenum). The antibody blocked IL-2-dependent spleen cell proliferation and nitric oxide production (Lee et al, 2011; pmid： 21872344).
Antibody First Published
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken interleukin‐2 rec eptor αlpha chain (CD25). Vet Immunol Immunopathol. 2011;144(3‐4)：396‐404. PMID：21872344
Note on publication
The paper describes the generation and characterization of the antibody, which is specific for chicken CD25.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
