Cat. No.
MABL-817
Application
WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
FMU-mCD226.1
From
Recombinant Antibody
Specificity
This antibody binds mouse CD226. This protein is expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. It is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. It acts as cell surface receptor for NECTIN2. On binding with ligand it stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG.
Alternative Names
DNAM1; DNAM-1; TLisA1; PTA-1; CD226 antigen; DNAX accessory molecule 1; T lineage specific activation antigen 1; platelet and T cell antigen 1
UniProt
Q8K4F0
Immunogen
The original antibody was generated by immunizing CD226KO (CD226 knockout) mice with purified CD226-Fc recombinant fusion protein.
Application Notes
The binding of this antibody to recombinant mouse CD226-Fc fusion protein was determined using indirect ELISA. This antibody is capable of binding mouse CD226 expressed on wild-type mouse platelets in a flow cytometric assay. This antibody was also reported to promote platelet aggregation. This antibody can be used to identify CD226 expressed in platelet lysates using western blot (CN111363042).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
