Cat. No.
MABL-750
Application
inhibit, WB, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
60.3
From
Recombinant Antibody
Specificity
This antibody binds the CD18 component of the β-2 integrin family which is expressed by leukocytes and neutrophils.
Alternative Names
ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA- 1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins
UniProt
P05107
Immunogen
The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.
Application Notes
This antibody is capable of inhibiting several cell mediated immune functions. It inhibits proliferative responses of T cells stimulated by soluble antigens, mitogens, or allogeneic cells in the initiation of a culture, but not when added after 48 hours. It successfully blocks the lytic activity of cytotoxic T cells and natural killer cells and the migration of neutrophilic granulocytes (PMID: 6644024). This antibody inhibits phagocytosis by reaction with the C3bi receptor, and stimulates phagocytosis by reverse opsonization (PMID: 3880787). This antibody inhibits aggregation of neutrophils in response phorbol myristate acetate, zymosan-activated plasma, and N-formyl-methionylleucylphenylalanine in a dose dependent manner (PMID: 3995185). 60.3 also inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo (PMID: 3539230). 60.3 successfully inhibits neutrophil function in vivo and has the potential to be used as an anti-inflammatory agent (PMID: 3693901). This antibody has application in blocking neutrophil mediated organ damage which occurs after myocardial infarct or hemorrhagic shock.Humanized version of this antibody binds CD18 with a 4-fold less affinity than its parent murine version (PMID: 7937713).
Antibody First Published
Beatty et al. Definition of a Common Leukocyte Cell-Surface Antigen (Lp95- 150) Associated With Diverse Cell-Mediated Immune Functions. J Immunol. (1983); 131(6):2913-8.PMID:6644024
Note on publication
Describes the generation and epitope mapping of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
