Cat. No.
MABL-706
Application
FACS, functional, IP, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-40
From
Recombinant Antibody
Specificity
OX-40 antibody binds specifically to CD4+ T blasts. CD134 is a protein of the TNFR superfamily. It binds to OX40 ligand of both the monomeric and trimeric states which results in co- stimulated proliferation.
Alternative Names
TNFRS4; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; OX40; MRC OX40; MRC OX-40; OX-40
UniProt
P15725
Immunogen
OX40 antibody was prepared by immunizing mice with phytohaemagglutinin-activated rat lymph node cells.
Application Notes
OX-40 antibody results in Fc-dependent (i.e. not Fab or F(ab')2) stimulation of in vitro activated T cell proliferation, but is not mitogenic for resting cells. This antibody can also label CD4+ T blasts and can pull down CD134/CD134+ cells.
Antibody First Published
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987 Dec;24(12):1281-90. PMID:2828930
Note on publication
Describes the identification of and functional studies using antibodies which can act as markers for activated T cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
