Cat. No.
MABL-680
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
33G5
From
Recombinant Antibody
Specificity
The antibody is specific for CD117. CD117 is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.
Alternative Names
Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; C-Kit receptor; CGMCCNO 21977;
UniProt
P10721
Immunogen
The original antibody was generated by immunizing mice with CD117.
Application Notes
The antibody specifically detected CD117 protein in Western blot analysis. The antibody 33G5 and the commercial antibody YR145 were used to stain gastrointestinal stromal tumor tissue. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 33G5 was higher than the commercial antibody. The antibody and the commercially available antibody YR145 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113621064A).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
