Cat. No.
MABL-662
Application
functional assays, ICC, IP, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
BZ5B8
From
Recombinant Antibody
Specificity
This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.
Alternative Names
CCR11; C-C chemokine receptor-like 2; Chemokine receptor CCR11; CKRX; CRAM-A; CRAM-B; G-protein coupled beta chemokine receptor; L-CCR; Lipopolysaccharide-inducible C-C chemokine receptor
UniProt
O35457
Immunogen
This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2- MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1–24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Application Notes
This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFα, IFN-γ, or poly
Antibody First Published
Zabel et al. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med. 2008 Sep 29;205(10):2207-20. PMID:18794339
Note on publication
Describe the original generation of the anti-mCCRL2 mAbs BZ5B8 and BZ2E3 and verification of their binding specificity for murine CCRL2 by an ELISA-based assay. The antibodies were then used as part of the study which suggested that mast cell–expressed orphan receptor CCRL2 bound chemerin and was required for optimal induction of IgE-mediated passive cutaneous anaphylaxis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
