Cat. No.
MABL-660
Application
neutralizing, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
9B1
From
Recombinant Antibody
Specificity
9B1 antibody recognizes CCR9 at the N-terminus. CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells.
Alternative Names
CD199; C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR-9; G- protein coupled receptor 28; GPR-9-6; CDw199; 9B-1
UniProt
Q9WUT7
Immunogen
This antibody was raised by immunizing rats with KLH-conjugated linear peptide corresponding to the N-terminus of mouse CCR9.
Application Notes
This antibody is recommended for the detection and analysis of CCR9 via flow cytometry (Farazuddin et al., 2019). Furthermore, it was demonstrated that this 9B1 antibody was capable of neutralizing CCR9 function, as it inhibited CCL25-induced calcium mobilization in L1.2-CCR9 cells (Rivera- Nieves et al., 2006).
Antibody First Published
Rivera-Nieves et al. Antibody blockade of CCL25/CCR9 ameliorates early but not late chronic murine ileitis. Gastroenterology. 2006 Nov;131(5):1518-29. Epub 2006 Aug 16.PMID:17101325
Note on publication
This article describes the generation and characterization of 9B1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
