Cat. No.
MABL-655
Application
IP, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
T21/8
From
Recombinant Antibody
Specificity
T21/8 antibody binds with high affinity to a CCR5 N-terminal epitope. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.
Alternative Names
CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor
UniProt
P51681
Immunogen
This antibody was raised by immunizing mice with intact RBL-CCR5 cells.
Application Notes
T21/8 antibody is recommedned for the detection and analysis of CCR5 via various methods. For instance, one group used this antibody to detect intracellular localization of CCR5 in RBL- CCR5 cells via immunofluorescence staining (Pollok-Kopp et al., 2003). Another group investigated ligand- mediated down-modulation of CCR5 via flow cytometry, utilizing T21/8 antibody (Fox et al., 2015). With other anti-CCR5 antibodies which react with CCR5 phosphorylated on different serine residues (Ab01578; Ab01579), it can be used to study dynamic regulation of CCR5 and phosphorylation mechanisms.
Antibody First Published
Pollok-Kopp et al. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. Epub 2002 Oct 27. PMID:12403770
Note on publication
This article describes the generation and characterization of the T21/8 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
