Cat. No.
MABL-530
Application
dotblot, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
BNYVV
Clone No.
14cG6
From
Recombinant Antibody
Specificity
Specific for epitope 4b (RTSP) which are amino acids 182-186 on the BNYVV coat protein. Binding is inhibited by large concentrations of plant material, and the epitope is readily cleaved in the presence of proteases.
Alternative Names
Beet necrotic yellowvein virus coat protein; BNYVV CP
UniProt
Q9YZK2
Immunogen
This antibody was raised by injecting purified BNYVV CP into mice.
Application Notes
This antibody is specific for epitope 4b of the BNYVV coat protein. Its binding specificity has been confirmed in dot blots (Commandeur et al, 1994) and western blots (Fecker et al, 1996) and double antibody sandwich ELISAs using an alkaline-phosphatase fusion protein (Uhde et al, 2000). It has been expressed as a scFv construct (Fecker et al, 1996) in both E. coli and higher plants.
Antibody First Published
Commandeur et. al. Location, Size, and Complexity of Epitopes on the Coat Protein of Beet Necrotic Yellow Vein Virus Studied by Means of Synthetic Overlapping Peptides Virology.1994 Jan;198(1)：282-7. PMID：7505070
Note on publication
Discusses the use of antibodies to analyse the different epitopes of the BNYVV coat protein using antibodies which recognise overlapping epitopes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
