Cat. No.
MABL-517
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Fab 19, actin
From
Recombinant Antibody
Specificity
This antibody is specific for the pointed end of actin filaments (Brawley et al, 2010; pmid: 21103060). Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed: 21103060). Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed: 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction.
Alternative Names
Actin, cytoplasmic 1; Fab 19; Fab19; Fab 19, actin; Actin, cytoplasmic 1; ACTB; Beta-actin
UniProt
P60709
Immunogen
The original Fab version of this antibody was obtained by recombinant antibody phage display.
Application Notes
Immunofluorescence was done with this antibody on actin filaments. In this experiment the antibodies bound to the pointed end of actin (Brawley et al, 2010; pmid: 21103060). ELISA was also preformed with this antibody on actin filaments. This was to confirm the binding of the antibody to actin filaments (Brawley et al, 2010; pmid: 21103060).
Antibody First Published
Brawley et al. Characterization of engineered actin binding proteins that control filament assembly and structure PLoS One. 2010 Nov 12;5(11):e13960. PMID:21103060
Note on publication
Describes the use of phage display mutagenesis to generate new class of binding protein that can be targeted to the pointed-end of actin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
