Cat. No.
MABL-470
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3B1D11A8
From
Recombinant Antibody
Specificity
This antibody binds human beta-catenin. The human β-catenin acts as a multi-functional protein that plays a very important role in cell adhesion and Wnt signaling pathway mediated gene expression regulation. Β-catenin on the cell membrane can be combined with the cytoplasmic domain of E- Cadherin (E-CAD) to form an e-cadherin / β-catenin complex, which mediates mutual adhesion between the same type. It acts as a key regulatory molecule in the Wnt signaling pathway and plays an important role in the occurrence / evolution of the embryonic development and tumor.
Alternative Names
CTNNB1; CTNNB; Catenin beta-1; Beta-catenin; CAT-5H10; CGMCC No. 19685
UniProt
P35222
Immunogen
The original antibody was generated by immunizing BALB/c mice with human beta-catenin- KLH conjugate in Freund's non-complete adjuvant.
Application Notes
The binding characterization of this antibody was done using ELISA. The original mouse IgG1 antibody binds human B-catenin with an affinity constant of 4.96 X 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of colorectal carcinoma and pancreatic cancer tissues. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody CAT-5H10 (CN112457400).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
