Cat. No.
MABL-468
Application
in vitro, SPR, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1E 8
From
Recombinant Antibody
Specificity
The antibody targets the central region of amyloid beta (residues 17–22).
Alternative Names
Aβ; CVAP; PN-II; Abeta; Amyloid-beta precursor protein; APP; ABPP; APPI; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide; PreA4; Protease nexin-II
UniProt
P05067
Immunogen
The original antibody was raised against residues 18–22 of amyloid beta.
Application Notes
The specificity of the original, Fab and scFv formats of the antibody was confirmed by ELISA analysis (Tammer et al., 2002; PMID: 12197886, Nisbet et al., 2013; PMID: 23766374). The original format of the antibody is extensively used for immunohistochemistry for detection of Aβ plaques. The original format and the Fab fragment bound to Ab[1–40/42/43] peptides by western blot analysis. The Fab format of the antibody shown to bind plaques within sections of human AD brain tissue by immunohistochemistry (Tammer et al., 2002; PMID:12197886). The scFv format of the antibody bound to the central region of Amyloid beta with an affinity of ∼55 nM and significantly reduced fibril formation of Amyloid beta 1–42. Furthermore, the scFv format of the antibody ameliorates Ab1–42-mediated toxicity in the PC12 cell line and murine primary neuronal cultures (Nisbet et al., 2013; PMID:23766374). The antibody was able to immunoprecipitate aliquots of individual EDTA-blood plasma samples from five different donors by magnetic bead immunoprecipitation (Klafki et al., 2020; PMID: 32911706).
Antibody First Published
Tammer et al., Generation of a recombinant Fab antibody reactive with the Alzheimer's disease-related Aβ peptide. Clin Exp Immunol. 2002 Sep; 129(3): 453–463. doi: 10.1046/j.1365- 2249.2002.01905.x PMID:12197886
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
