Cat. No.
MABL-454
Application
crystallography, IP, Neutralizing;SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Astrovirus 2
Clone No.
PL-2
From
Recombinant Antibody
Specificity
The antibody binds to the surface of the HAstV-2 virion, which is formed by the virus capsid protein. The antibody binds to a conformation-dependent quaternary epitope on each side of the dimeric capsid spike. Strains of HAstV-2 (HAstV-2-Oxford, -RIVMa, -RIVMb, and -RIVMc) are resistant to neutralization by the antibody.
Alternative Names
Human astrovirus 2; HAstV-2; Capsid polyprotein VP90; Capsid protein; HAstV-2 capsid spike protein
UniProt
Q82446
Immunogen
The original antibody was isolated by immunizing BALB/c mice with H-Ast-2 virus.
Application Notes
The original antibody (IgG2a) was used for immunofluorescence staining of H-Ast2- infected LLCMK2 cells. The antibody neutralizes HAstV serotype 2 (Sanchez-Fauquier et al., 1994; PMID: 7514320). The original antibody (IgG2a) and the scFv fragment bound to wheat germ extract containing recombinant HAstV-2 capsid protein, as confirmed by ELISA analysis. The crystal structure of the Fab fragment was determined. The scFv fragment was used to immunoprecipitate HAstV-2 capsid protein. This antibody could potentially be developed as a therapeutic and diagnostic agent (Bogdanoff et al., 2016; PMID: 27213181). The specificity of the antibody and scFv fragment for the HAstV-2 CP spike were confirmed by ELISA. The binding affinity of the Fab and scFv fragments towards the spike protein was found to be nearly identical (KD of 1.8 nM) using surface plasmon resonance. The structure of the HAstV capsid spike domain bound to the neutralizing scFv fragment was determined. The antibody blocked the attachment of recombinant HAstV-2 CP spike to Caco-2 cells supporting the hypothesis the antibody neutralizes HAstV by blocking virus attachment to cells (Bogdanoff et al., 2016; PMID: 27807234).
Antibody First Published
Sanchez-Fauquier et al. Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization Virology. 1994 Jun;201(2):312-20. doi: 10.1006/viro.1994.1296. PMID:7514320
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
