Cat. No.
MABL-447
Application
crystallography, in vitro, in vivo, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
36-65
From
Recombinant Antibody
Specificity
The antibody is specific for arsonate.
Alternative Names
arsonate(1-); hydrogen arsonate; CHEBI: 64448; Q27133288; hydroxyarsinate
UniProt
Immunogen
The original antibody was generated by immunization A/J mice with the hapten p- azophenylarsonate.
Application Notes
The antibody is a relatively low-affinity germline-encoded antibody with a true Ka of 2.5*10^5 M-1 (Rothstein et al., 1983; PMID: 6843553). The specificity of the IgG1 format of the antibody was confirmed by ELISA analysis. The antibody was employed in in vitro binding assay, the antibody bound effectively to high density antigen beads with retention of 57.8% while the binding to low density target was 17.6%. The antibody behavior in vivo,using antigen-substituted beads s.c. implanted into SCID mice was examined. Results showed the antibody bound effectively to the HD antigen target (Zuckier et al., 2000; PMID: 11156404). The crystal structure of the Fab fragment was determined and the antibody affinity for the hapten was measured (Kd= 8.1 mM) (Sethi et al., 2006; PMID: 16618601).
Antibody First Published
Siekevitz et al. The genetic basis of antibody production the dominant anti- arsonate idiotype response of the strain A mouse. Eur J Immunol. 1982 Dec;12(12):1023-32. PMID:6186498
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
