Cat. No.
MABL-421
Application
electron microscopy, therapeutic, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Cryptosporidium parvum
Clone No.
3E 2
From
Recombinant Antibody
Specificity
This antibody binds the apical complex glycoprotein CSL of Cryptosporidium parvum. The apicomplexan protozoan Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences.
Alternative Names
CSL; αCSL; Apical complex glycoprotein, CSL (circumsporozoite-like) antigen
UniProt
Immunogen
The original antibody was generated by immunizing mice with GP25-200 of Cryptosporidium parvum.
Application Notes
Apical complex glycoprotein antigen of Cryptosporidium parvum is critical to infectivity and targets protective immunity. This antibody exhibited morphologic changes in sporozoites and merozoites characterized by rapid and progressive formation, posterior movement, and release of membranous Ag-mAb precipitates. This antibody was capable of recognizing 46-770 kDa sporozoite antigens and 1300 kDa antigen soluble glycoprotein exoantigen (CSL) expressed by merozoites in a western blot (PMID: 9029117). This antibody elicited circumsporozoite precipitate (CSP)-like reaction which is characterized by the progressive posterior formation and release of membranous antigen-MAb complexes, after which zoites are rendered noninfective. This antibody is capable of neutralizing C. parvum in vitro and also capable of passively protecting against oocyst challenge and infection in vivo. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 10768951). This antibody was reported to be highly effective in in reducing, but not eliminating, persistent C. parvum infection (PMID: 11796330). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143).
Antibody First Published
Riggs et al. Protective monoclonal antibody defines a circumsporozoite-like glycoprotein exoantigen of Cryptosporidium parvum sporozoites and merozoites. J Immunol. 1997 Feb 15;158(4):1787-95. PMID:9029117
Note on publication
Describes the generation of neutralizing antibodies against C. parvum and their role in providing passive immunization against cryptosporidiosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
