Cat. No.
MABL-393
Application
WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
EM22703
From
Recombinant Antibody
Specificity
This antibody is specific for the extracellular domain of human recombinant transmembranetruncated is also involved in tumour growth and angiogenesis.
Alternative Names
αvβ3; Vitronectin receptor subunit alpha; CD51; ITGB3; Platelet membrane glycoprotein IIIa; CD61; P06756; P05106; avb3
UniProt
Immunogen
This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas
Application Notes
This antibody has been used to specifically bind αvβ3 in Western blots and in flow cytometry, and to specifically stain αvβ3 integrins in FFPE (formalin fixed paraffin embedded)/TMA material,where murine antibodies function poorly (Goodman et al, 2012; Heß et al., 2014; Roth et al, 2013). αvβ3 was found to be strongly expressed in some invasive NSCLC samples, though was not expressed by PC3 prostate cells and human prostate tumours. In FC, the EM22703 signal is increased 3–10 fold in the presence of nanomolar concentrations of the antagonistic αvβ3 inhibitor cilengitide, indicating that EM22703 detects a ligand-induced binding site on αvβ3 (Goodman et al, 2012).
Antibody First Published
Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423
Note on publication
Describes the generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
