Cat. No.
MABL-391
Application
NTRL, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Clostridium perfringens
Clone No.
3A4D10
From
Recombinant Antibody
Specificity
This antibody binds the alpha toxin of Clostridium perfringens. It specifically recognizes and binds the epitope 'ARGFAK' of the toxin.
Alternative Names
PLC; Phospolipase C; Zn-metallo phospholipase C; Alpha-toxin;Hemolysin; Lecithinase; Phosphatidylcholine cholinephosphohydrolase; α-Toxin
UniProt
Q0TV31
Immunogen
The orignal antibody was generated by immunizing BALB/c mice with an immunogenic toxoid prepared by incubating the alpha toxin with formaldehyde.
Application Notes
Alpha toxin is an exotoxin, a type of bacterial haemolysin that attack blood cell membranes and cause cell rupture. Alpha toxin is an essential virulance factor in gas gangrene and causes myonecrosis in infected tissues. ELISA was used to identify the specific clones binding to alpha-toxin and epitope mapping of the antibody. 3A4D10 can even recognize denatrued toxin in a western blot assay. This antibody is capable of neutralizing the toxin in vitro and in vivo assays (PMID: 1718874). Only immunization with amino acids 247-370 of the Clostridium perfringens alpha toxin induced protection against the lethal effects of the toxin. The toxin plays a major role in pathogenesis of gas gangrene (PMID: 825650).Humanized version derived from its murine parent showed higher binding affinity (PMID: 7716162).
Antibody First Published
Logan et al. Epitope Mapping of the Alpha-Toxin of Clostridium perfringens.Infect Immun. (1991); 59(12): 4338–4342. PMID:1718874
Note on publication
Describes the generation and epitope mapping of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
