Cat. No.
MABL-349
Application
crystallization, inhibition, RIA, SPR
Isotype
Engineer antibody
Species Reactivity
Electrophorus electricus
Clone No.
Elec408
From
Recombinant Antibody
Specificity
The antibody is specific for EeAChE, and it targets an epitope located in the backdoor region, distant from the gorge entrance. The antibody does not cross react with human AChE, bovine AChE, Torpedo AChE, and snake AChE or denatured AChE. The antibody binds the EeAChE subunit non_x0002_competitively with PAS ligands Fas2, Elec403 and Elec410.
Alternative Names
AChE; EC 3.1.1.7
UniProt
O42275
Immunogen
The original antibody was generated by immunizing mice against Electrophorus electricus acetylcholinesterase.
Application Notes
The original antibody (IgG1) bound AChE with high affinity and strongly inhibited the antigen with an apparent Ki value less than 0.1 nM. The relative affinity of the antibody for AChE was evaluated using a competitive radioimmunoassay (Kd= 38 pM). The inhibition of E. electricus AChE by the antibody was studied as a function of substrate concentration, the inhibition obtained with the antibody was about 75 % (Remy et al., 1995; PMID: 7649165). The epitope mapping was studied by site-directed mutagenesis, showing the antibody binds to the AChE back door (Simon et al., 1999; PMID: 10488117). The structure of the Fab fragment was determined by X-ray (Bourne et al., 2013; PMID: 24146971).
Antibody First Published
Remy et al. Characterization of monoclonal antibodies that strongly inhibit Electrophorus electricus acetylcholinesterase. Eur J Biochem 231: 651-658. PMID:7649165
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
