Cat. No.
MABL-347
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10D
From
Recombinant Antibody
Specificity
This antibody binds human acetylcholinesterase, which is a cholinergic enzyme primarily found at postsynaptic neuromuscular junctions, especially in muscles and nerves. It immediately breaks down or hydrolyzes acetylcholine (ACh), a naturally occurring neurotransmitter, into acetic acid and choline.
Alternative Names
ACHE; AChE; HuAChE
UniProt
P22303
Immunogen
The original mouse antibody was generated by immunizing mice with full-length recombinant human acetylcholinesterase (rHuAChE) as immunogen.
Application Notes
Acetylcholinesterase is an enzyme that catalyzes the breakdown of acetylcholine and of some other choline esters that function as neurotransmitters. The original mouse IgG1 version of this antibody binds human acetylcholinesterase with a binding affinity of 0.4 nM. The binding affinity of mouse IgG1 version for truncated, monomeric recombinant HuAChE was measured by ELISA. This antibody was used in the immunopurification of membrane bound AChE on human RBCs by binding on immobilized antibody for further detection by mass spectrometry. RBC AChE can be used as a biomarker for exposure to organophosphorus pesticides and nerve agents (PMID: 28892361).
Antibody First Published
Dafferner et al. Immunopurification of acetylcholinesterase from red blood cells for detection of nerve agent exposure. Chem Res Toxicol. 2017 Oct 16;30(10):1897-1910. PMID:28892361
Note on publication
Describes the generation and utilization of this antibody in immunopurification of human RBC AChE for further detection by mass spectrometry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
