Cat. No.
MABL-339
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Bordetella pertussis
Clone No.
2F5
From
Recombinant Antibody
Specificity
This antibody is specific for the glycine and aspartate-rich nonapeptide repeat region (amino acids 1156-1489) of Bordetella pertussis adenylate cyclase toxin.
Alternative Names
AC; Bordetella pertussis adenylate cyclase toxin; adenylate cyclase toxin
UniProt
Immunogen
The mouse version of this antibody was generated by immunizing BALB/c mice with Bordetella pertussis adenylate cyclase toxin purified using sucrose gradient centrifugation.
Application Notes
The mouse version of this antibody was used for immunoblotting on different epitopes of the adenylate cyclase toxin from Bordetella pertussis. It was most sensitive to amino acids 1156-1489 (Lee et al, 1999; pmid:10225859). An ELISA was preformed on purified AC toxin using the mouse version of this antibody. The binding of the mouse version of this antibody was assessed at different calcium concentrations. It showed only a small decrease in binding affinity at high calcium concentration (Hewlett et al, 1991; pmid:1894634).
Antibody First Published
Hewlett et al. Adenylate cyclase toxin from Bordetella pertussis. Identification and purification of the holotoxin molecule J Biol Chem. 1989 Nov 15;264(32):19379-84. PMID:2553737
Note on publication
Bordetella pertussis adenylate cyclase (AC) toxin is a calmodulin-activated adenylate cyclase enzyme which has the capacity to enter eukaryotic target cells and catalyze the conversion of endogenous ATP into cyclic AMP. In this work, the AC holotoxin molecule is identified and isolated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
