Cat. No.
MABL-315
Application
agonist, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BBK4
From
Recombinant Antibody
Specificity
This antibody is specific for human CD137.
Alternative Names
CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9; TNFRSF9; T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; BBK-4
UniProt
Q07011
Immunogen
The original antibody was raised by immunizing a mouse with CD137.
Application Notes
This antibody stimulates T-cell activity (Son et al, 2004; pmid:15087232). ELISA was performed using this antibody on E.coli expressed human 4-1BB (Son et al, 2004; pmid:15087232). Mixed lymphocyte reaction was investigated with fresh peripheral blood mononuclear cells using this antibody (Son et al, 2004; pmid:15087232). This antibody was used for flow cytometry on a subline of human Jurkat 8-1 cells (Son et al, 2004; pmid:15087232). This antibody promoted long-term proliferation of PBMC (Son et al, 2004; pmid:15087232).
Antibody First Published
Son et al. Humanization of agonistic anti-human 4-1BB monoclonal antibody using a phage-displayed combinatorial library. J Immunol Methods. 2004 Mar;286(1-2):187-201. PMID:15087232
Note on publication
The humanization of agonistic anti-human 4-1BB mAb, BBK-4, using a phage display library.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
