Cat. No.
MABL-300
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
S48-18
From
Recombinant Antibody
Specificity
This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).
Alternative Names
UniProt
Immunogen
This antibody was generated by immunizing BALB/c mice with αKdo(2→4)αKdo(2→4)αKdo_x0002_BSA.
Application Notes
An ELISA was performed on neoglycoconjugates using the mouse version of this antibody, it showed the strongest binding to αKdo(2→4)αKdo(2→4)αKdo and αKdo(2→4)αKdo(2→4)αKdo(2→6)βGlcNAc. ELISA was also performed on LPS antigens and bacterial antigens using the mouse version of this antibody, it bound most strongly to Chl. psittaci EB and Chl. psittaci LPS (Brade et al, 2000; pmid:11521056). The scFv version of this antibody was used for an ELISA on neoglycoconugates, the antibody bound to 2.4/2.4PS4P and HS4P quite well but bound less well to 2.8/2.4PS4P (Gerstenbruch et al, 2010; pmid:20022906).
Antibody First Published
Brade et al. A monoclonal antibody recognizing the 3-deoxy-D-manno-oct-2- ulosonic acid (Kdo) trisaccharide alphaKdo(2-->4)alphaKdo(2-->4)alphaKdo of Chlamydophila psittaci 6BC lipopolysaccharide. J Endotoxin Res. 2000;6(5):361-8. PMID:11521056
Note on publication
A monoclonal antibody (mAb) S45-18 was generated against a synthetic neoglycoconjugate containing the trisaccharide alphaKdo(2-->4)alphaKdo(2-->4)alphaKdo (Kdo, 3-deoxy-D_x0002_manno-oct-2-ulopyranosonic acid) which represents a structure of the lipopolysaccharide (LPS) from Chlamydophila psittaci 6BC.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
