Cat. No.
MABL-288
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Fusarium species
Clone No.
NAT-267
From
Recombinant Antibody
Specificity
This antibody is specific for mycotoxin 15-acetyldeoxynivalenol (15-AcDON). 15- Acetyldeoxynivalenol belongs to the class of type B trichothecene mycotoxins, generated by several Fusarium fungi. Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15- acetyldeoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock.
Alternative Names
15-AcDON; DON; Mycotoxin; Deoxynivalenol; Vomitoxin, Fusarium mycotoxin; mNAT-267; NAT-267; ChEBI: 10022
UniProt
Immunogen
The original antibody was generated by immunization of a llama (Llama glama) with the protein conjugate 15-DON-BSA in a TiterMax Classic adjuvant.
Application Notes
This antibody can bind 15-AcDON in a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) and fluorescence polarization assay (FPA). This antibody does not cross react with DON; 3-acetyldeoxynivalenol (3-AcDON); neosolaniol (NEO); diacetoxyscirpenol (DAS); and T-2 toxin. Surface plasmon resonance (SPR) analysis of soluble NAT-267 binding kinetics to immobilized 15- DON-Horse Radish Peroxidase (HRP) indicated a dissociation constant KD of 5microM (PMID: 18632156).
Antibody First Published
Doyle et al. Cloning, expression, and characterization of a single-domain antibody fragment with affinity for 15-acetyl-deoxynivalenol. Mol Immunol. 2008 Aug;45(14):3703-13. PMID:18632156
Note on publication
Describes the generation of a llama single-domain antibody fragment specific to the mycotoxin 15-acetyldeoxynivalenol.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
