Key features and details | |
Cat. No. | MABL-774 |
Name | Anti-CD2 mAbs |
Clone No. | AFD- OX-34 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, FC |
Species Reactivity | Rat |
Basic Information | |
Specificity | OX-34 binds specifically to rat CD2. CD2 is type I membrane protein involved in triggering of T- cells (cytoplasmic domain is involved in signaling function) and is expressed on thymocytes, peripheral T cells, dendritic epidermal T cells, macrophages in the spleen, and NK cells. |
Alternative Name | LFA-2; LFA2; OX-34 antigen; OX34 antigen; T-cell surface antigen T11/Leu5; T cell surface antigen T11/Leu5; OX34; MRC OX34; MRC OX-34 |
UniProt | P08921 |
Immunogen | OX-34 was prepared by immunizing mice with T blasts prepared in mixed lymphocyte reactions with purified rat T helpercells against irradiated spleen. |
Application Notes | Ox34 blocks CD48 binding to CD2 causing partial block of T cell activation; OX34 can be used to deplete peripheral T cells (used to prevent allograft rejection in vivo). |
Antibody First Published | Arvieux et al. Monoclonal antibodies against a rat leucocyte antigen block antigen-induced T-cell responses via an effect on accessory cells. Immunology 58：337 1986 PMID：2942461 |
Note on publication | Describes the use of OX-45 in blocking the primary mixed lymphocyte response (MLR)and the secondary response of T lymphocytes to the soluble antigen DNP-BGG. It also showed that OX- 45 did not have any effect on the cytotoxic activity of specific (CTL) or non-specific (NK) killer cells or on proliferative responses stimulated by lectins or oxidative mitogenesis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
