Cat. No.
MABL-280
Application
Catalytic assays, crystallization
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
33F12
From
Recombinant Antibody
Specificity
This antibody was raised against 1,3-diketone hapten, but it is capable of catalyzing the decarboxylation of structurally related β-keto acids.
Alternative Names
Hapten 1,3-diketone; β-keto acids; beta keto acids
UniProt
Immunogen
The original antibody was generated by immunizing GIX+ mice with a keyhole limpet hemocyanin (KLH)-coupled diketone hapten.
Application Notes
This antibody efficiently catalyzes aldol and retro-aldol reactions and covalently binds 1,3-diketone hapten. The (kcat/KM )/kuncat is approximately 109 at pH 7.5 as measured by the consumption of substrates and production of aldol products (monitored using reversed-phase high_x0002_performance liquid chromatography (HPLC) and enzymatic assays) (Wagner et al., 1995; PMID: 8525368). This antibody efficiently catalyzed the decarboxylation of two hapten-related β-keto acids (marked 4 and 5 in scheme 1 in the publication) with significant rate enhancements (kcat/kuncat) of 4959 and 11,261, respectively (Björnestedt et al., 1996). The crystallization of the Fab version of this antibody revealed a binding pocket characterized by a narrow, elongated cleft, where the catalytic lysine residue is prominently located (Barbas III et al., 1997; PMID: 9405338).
Antibody First Published
Wagner et al. Efficient aldolase catalytic antibodies that use the enamine mechanism of natural enzymes Science. 1995 Dec 15;270(5243):1797-800. doi: 10.1126/science.270.5243.1797 PMID:8525368
Note on publication
The original publication describes the generation of efficient aldolase catalytic antibodies that utilize the enamine mechanism of natural enzymes, mimicking their catalytic process and exhibiting diastereofacial selectivity in the aldol reaction.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
